pRARE-MBP-DEST - Addgene
WEBPlasmid pRARE-MBP-DEST from Dr. Neil Olszewski's lab is published in Plant Cell. 2012 Jan;24(1):96-108. doi: 10.1105/tpc.111.093518. Epub 2012 Jan 20. This plasmid is available through Addgene.
Introduction of plasmid encoding for rare tRNAs reduces …
WEBHowever, phagemid vectors that utilize Cam as a selection marker would require swapping of antibiotic resistance in pRARE or the phagemid before use. Here we present a simple method to reduce amplification bias in phage display protocols by the addition of a plasmid coding for rare tRNAs.
Plasmid pRARE as a vector for cloning to construct a ... - PubMed
WEBAbstract. The gene of methylase M.SccL1I that protects DNA against hydrolysis with the nickase N.BspD6I was inserted into plasmid pRARE carrying genes of tRNA, which are rare in E. coli.
Plasmid pRARE essentially constructed in Rosetta, it enables …
WEBDownload scientific diagram | Plasmid pRARE essentially constructed in Rosetta, it enables Rosetta for the expression of protein often get fails in conventional expression host system.
pQTEV-TPT1 - Addgene
WEBThe vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins.
Harnessing stepping-stone hosts to engineer, select, and reboot ...
WEBMay 23, 2022 — DH10B/pRARE was constructed via transforming chloramphenicol resistant plasmid pRARE (Umlauf et al., 2015) that encodes six rare tRNAs into DH10B.
Introduction of Plasmid Encoding for Rare tRNAs Reduces …
WEBApr 3, 2018 — K91 E. coli cells are transformed with pRARE, which encodes for expression of 6 rare tRNAs and imparts chloramphenicol resistance. Once the cells are transformed, phage amplification occurs by standard protocols using chloramphenicol and tetracycline as section markers.
WEBplasmid pRARE carrying genes of transfer RNAs rare for Escherichia coli. MATERIALS AND METHODS In this work we used plasmids pET28b, pRARE (Novagen, USA), p15MSsc [8] and E. coli strain TOP10F′: F′ [lacIq, Tn10(TetR) mcrA ∆(mrrhsdRMS Biochemistry (Moscow), Vol. 69, No. 10, 2004, pp. 11231127.
Plasmid pRARE as a Vector for Cloning to Construct a ... - Springer
WEBAbstract. The gene of methylase M. Scc L1I that protects DNA against hydrolysis with the nickase N. Bsp D6I was inserted into plasmid pRARE carrying genes of tRNA, which are rare in E. coli.
Plasmid pRARE as a vector for cloning to construct a …
WEBOct 1, 2004 — The gene of methylase M.SccL1I that protects DNA against hydrolysis with the nickase N.BspD6I was inserted into plasmid pRARE carrying genes of tRNA, which are rare in E. coli.
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