when one plasmid contains a library), we prepare electrocompetent cells containing the constant partner. Figure 2: (a) Schematic plate layout for screening interactions with the GFP reassembly screen.

The gene coding for GFP comes from the bioluminescent jellyfish, Aequorea victoria, and resides on a DNA plasmid called pGLO. The pGLO plasmid also contains a gene encoding for beta-lactamase.

The Global Field Program (GFP) is a master’s degree program that brings scientists, educators, community leaders, and others together at critical conservation hotspots in Africa, Asia, Australia, and ...

Our modern relationship with plastics is complicated. The material is ubiquitous in packaging and product design, but plastic ...

Transcription activation of genes by estrogens is driven by enhancers, which are often located within the same Topologically Associating Domain (TAD) as non-targeted promoters. We investigated how ...

This approach involved transforming CRISPR-Cas9 ribonucleoprotein complexes directly instead of encoding single-guide RNAs (sgRNAs) and Cas9 from a plasmid. Moreover ... integrated green fluorescent ...

This important manuscript provides insights into the competition between Splicing Factor 1 (SF1) and Quaking (QKI) for binding at the ACUAA branch point sequence in a model intron, regulating exon ...