An equal amount of stop buffer (95% formamide) was added ... for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines 52 using Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712) ...

All cell lines underwent regular testing to confirm their being free of mycoplasma contamination, using mycoplasma PCR detection (TaKaRa Taq Version 2.0 ... Cells were lysed in RIPA buffer containing ...

PCR mixture (50 uL) contained 5 µl of DNA template (~5 ng), 5 µl of 10× AccuPrime™ PCR Buffer II, 0.2 µM of each primer, and 1 U of AccuPrime™ Taq DNA Polymerase High Fidelity (Life Technologies). The ...

1× reaction buffer, 1.5 mM MgCl 2, 200 µM dNTPs, 10 pmol of each primer (Mbcr1: 5′-GAAGTGTTTCAGAAGCTTCTC-3′; 2oabl1: 5′-TGATTAAGCCTAAGACCC GGA-3′; mbcr1: 5′-CCATCGTGGGCGTCCGCA-3′), 0.5 U Taq DNA ...

PCR reactions were performed in a final volume of 10 µL containing 20 ng of genomic DNA, 1X PCR Buffer (Roche, Woerden ... and 0.5 U FastStart PCR Taq DNA Polymerase (Roche). The thermal profile was ...

Subsequent chromatographic separation was performed on a reverse phase nanospray column (Thermo Scientific EASYnano-LC, 3 μm, 75 μm ID × 100 mm C18 column) using a 30 minute linear gradient from 10% - ...

JERUSALEM — Prime Minister Benjamin Netanyahu said Tuesday that Israeli forces will stay in a buffer zone on the Syrian border, seized after the ouster of Syrian President Bashar Assad ...

Israel entered the demilitarized buffer zone, to the east of the Golan Heights, earlier this month following the collapse of the Bashar al-Assad regime. Its capture of about 400 square ...

Theo Panayides interviewed the dozens of migrants stuck in the buffer zone several times and realised a hard truth about journalism I wrote a number of stories in 2024 that meant a lot to me (the ...

The U.N. special envoy to Syria on Wednesday gave one of his first signs of optimism about Syria’s new government as the de facto leaders, the Islamist rebel group Hay’at Tahrir al-Sham, work ...