Bacteriophage P1 does not show spatial preference when infecting
By fluorescently labeling P1 virions, we found that P1 shows no spatial preference on cell surface adsorption. In addition, adsorption sites do not appear to affect the success rate for P1 to inject its DNA into the cell through our live-cell phage DNA imaging.
Bacteriophage P1 does not show spatial preference when infecting
Mar 1, 2020 · By fluorescently labeling P1 virions, we found that P1 shows no spatial preference on cell surface adsorption. In addition, live-cell phage DNA imaging revealed that adsorption sites do not affect the success rate for P1 in injecting its DNA into the cell.
P1 phage - Wikipedia
P1 is a temperate bacteriophage that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle the phage genome exists as a plasmid in the bacterium [1] unlike other phages (e.g. the lambda phage) that integrate into the host DNA.
Microscopic phage adsorption assay: High-throughput …
In the current study, we develop and validate a Microscopic Phage Adsorption (MPA) assay as a method to quantify phage adsorption dynamics at single-virus resolution. We combined widefield fluorescence microscopy to visualize individual phage …
Visualization of bacteriophage P1 infection by cryo-electron …
3D reconstructions reveal phage P1 infecting tiny E. coli at an m.o.i of ~1 after 10–30 min of incubation. (A, B) three contracted phages and one extended phage interact with one E. coli minicell. (C) Another minicell with two contracted phages.
9.7G: Temperate Bacteriophages - Lambda and P1 - Biology …
Nov 23, 2024 · Temperate phage, such as P1, have the ability to exist within the bacterial cell they infect in two different ways. In lysogeny, P1 can exist within a bacterial cell as a circular DNA, in that it exists by replicating as if it were a plasmid and does not cause cell death.
Phage microscopy: The Types, Techniques, and Importance
Jan 27, 2023 · Understanding Phage Structure: By visualizing phages under a microscope, scientists can study the physical structure of these viruses in great detail. This information is crucial for understanding how phages infect and replicate within their host cells.
Interactions between Viral Regulatory Proteins Ensure an MOI ...
Sep 14, 2021 · Phage P1 is a temperate phage which makes the lytic or lysogenic decision upon infecting bacteria. During the lytic cycle, progeny phages are produced and the cell lyses, and in the lysogenic cycle, P1 DNA exists as a low-copy-number plasmid and replicates autonomously.
Under the Microscope Bacteriophages or “phages” are viruses of prokaryotes. At least 5,360 tailed and 179 cubic, filamentous, and pleomorphic bacterial viruses have been examined in the electron microscope since the introduction of negative staining in 1959. Since at least 100 novel bacterial viruses
P1 Ref Endonuclease: A Molecular Mechanism for Phage …
Jan 14, 2016 · Ref is a P1 function that amplifies the lytic cycle under conditions when the bacterial SOS response is induced due to DNA damage. The effect of Ref is multifaceted. DNA binding by Ref interferes with normal DNA metabolism, and the nuclease activity of Ref enhances genome degradation.
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