Pilot scale testing and modeling of enzymatic reactive absorption …
Jul 1, 2017 · Enzymatic reactive absorption (ERA) with dissolved and immobilized carbonic anhydrase was demonstrated in pilot scale packed columns. ERA leads to substantial enhancement of CO 2 absorption into aqueous MDEA solutions. Similar ERA rates measured for 20 and 40 °C, believed to result from compensation of competing physical and chemical effects.
Pilot-scale production of a highly thermostable α-amylase enzyme …
Enzyme activity was analyzed using the DNS (dinitrosalicylic acid) method 30 by determination of the reducing sugars. Maltose was used as a standard to measure the liberated reducing sugars in a spectrophotometer at 550 nm.
Pilot-scale production and in-situ application of petroleum …
Feb 22, 2022 · Alcanivorax borkumensis is an alkane-degrading bacteria that can produce petroleum degrading enzymes such as alkane hydroxylase and lipase. In this study, pilot-scale Alcanivorax borkumensis fermentation was developed for producing large volumes of petroleum degrading enzymes cocktail (∼900 L).
Pilot scale absorption experiments with carbonic anhydrase-enhanced ...
Mar 1, 2019 · This study demonstrates how the enzyme enhanced CO 2 capture process in pilot scale benchmarks to the industrial standard solvent 30 wt% MEA.
Evaluation of scale-up effect on cold-active enzyme production …
A novel cold-adapted bacteria (Arthrobacter sp. S2TR-06) was isolated that could produce cold-active degradative enzymes (xylene monooxygenase (XMO) and catechol 2,3-dioxygenase (C2,3D)). Enzyme production was investigated on 4 different scales (lab to pilot scale).
Evaluation of scale-up effect on cold-active enzyme production …
May 15, 2023 · The pilot scale bioreactor (150 L) had the highest production of enzymes and biomass (107 g/L for biomass, 109 U/mL for XMO, and 203 U/mL for C2,3D after 24 h) due to enhanced oxygenation. Our results showed that adding FeSO 4 at 0.1% before extraction of membrane-bound enzymes can enhance the activity of …
Screening, Optimization and Process Scale up for Pilot Scale …
The lipase production was scaled up on a pilot scale in a 5 Liter fermenter maintaining growth parameters of pH 7, temperature at 28°C, stirrer rate at 120 rpm, airflow rate at 30 L/hr, O2 saturation 50% and pressure 0.05 MPa. The crude enzyme was extracted for further assays.
(PDF) Pilot-Scale Evaluation of Enzyme Pre-Treatment to Enhance ...
In order to assess practical application, enzyme pre-treatment was applied at the Wilmington wastewater treatment plant, using a pilot-scale centrifuge. However, the dewatering efficiency reached in laboratory scale could not be obtained in pilot scale, where the final cake solids content reached only 20% with different enzyme doses.
Pilot scale testing and modeling of enzymatic reactive absorption …
Jul 1, 2017 · This paper provides an industrial-scale technical assessment of absorption of CO2 in water to react into bicarbonate (HCO3−), with the goal of storing HCO3− in the oceans as a carbon sequestration …
Prediction of the pilot-scale recovery of a recombinant yeast enzyme …
Jul 20, 2001 · This article describes the rapid prediction of recovery process performance for a new recombinant enzyme product on the basis of a broad portfolio of computer models and highly targeted experimentation.