pUC19 - Wikipedia
pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. [1] The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. [2] The pUC plasmids are all circular double ...
pUC19 Vector- Definition, Structure, Sites, Applications - Microbe …
Aug 3, 2023 · pUC plasmids were first developed by Joachim Messing and his co-workers. It is a commonly used cloning vector in the bacteria E. coli. pUC19 is 2686 bp in length. The molecular weight of the pUC19 vector is 1.75×10 6 Da. It is a small plasmid with a high copy number.
pUC19 Sequence and Map - SnapGene
pUC19. Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18.
pUC19 Vector - NEB
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
3.4: Prokaryotic Expression Vectors - Biology LibreTexts
The first vector we will consider is the pUC family of vectors. Figure 3.4.1: pUC vector. Although it is not typically used for the expression of recombinant proteins, it has all the necessary elements of an expression vector: An origin of replication. The pUC family of vectors are high copy vectors.
pUC19 - Addgene
Vector Database. Browse a digital-only collection of vector backbone information
pUC57 Sequence and Map - SnapGene
Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions.
Cloning vector - Wikipedia
A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. [1] ... therefore cloning large fragments may require more specialised cloning vector. [6] The pUC plasmid has a high copy number, contains a multiple cloning site (polylinker), a ...
pUC18 – Probably the Best High-Copy plasmid in the World!
Jan 16, 2020 · pUC18 is one of the most widely used cloning and expression vectors within research labs today. The pUC series (containing pUC18, 19) was created at the University of California by Yanisch-Perron et al. as a modification of an existing group of cloning plasmids.
pUC Vector: Features, Selectable markers, Blue White Screening …
What is a pUC vector? Vector obtained by modifying pBR 322 vector. Smaller in size 2686bp with high copy number. ‘p’=plasmid “UC”: University of California. Joachim Messing and co-workers (1983) Regions: 1. Ori: E.coli origin. 2 selectable markers: ampicillin (amp r)& lacZ’ gene. This region has genes providing resistance against ampicillin,