Pegylated recombinant human epidermal growth factor (rhEGF…
Jun 1, 2002 · Recombinant human epidermal growth factor (rhEGF) was conjugated with polyethylene glycol (PEG) to improve its physical stability during microencapsulation in biodegradable poly (lactic-co-glycolic acid) microspheres. rhEGF was conjugated with N -hydroxysuccimide (NHS)-derivatized methoxy-PEG (mPEG) of …
Effect of molecular size of PEGylated recombinant human …
The purpose of this study was to investigate the effect of size of polyethylene glycol (PEG) conjugated to recombinant human epidermal growth factor (rhEGF) on its stability in skin wound tissue and in vitro biological activity to find the desirable conjugate as …
Screening for stability and compatibility conditions of recombinant ...
Aug 16, 2013 · Most rhEGF–excipient instability occurs among this protein and reducing sugars. Polymers like poly(ethylene glycol) (PEG) and polysorbates increased methionine oxidation. The rhEGF oxidation and deamidation were the most common degradation pathways.
Effect of Molecular Size of PEGylated Recombinant Human Epidermal ...
Oct 7, 2008 · This study suggests that the high molecular weight PEG at N-terminus of rhEGF would give a satisfactory stabilizing effect and thus may improve therapeutic efficacy in clinical use. Keywords: recombinant human epidermal growth factor , PEGylation , protein stability , biological activity , wound healing.
Recombinant human epidermal growth factor (rhEGF) stimulates endogenous neural precursor cells in the subventricular zone (SVZ) leading to tissue regeneration in animal models of stroke1.
Effect of molecular size of PEGylated recombinant human …
Site-specific PEGylation at N-terminus of rhEGF was performed with monomethoxy PEG-Butyraldehyde derivatives (MW 2, 5, and 20 kDa). Mono-PEG-rhEGFs retained 60-70% of biological activity of native rhEGF, and the effect of PEG size was not significant.
Polyethylene glycol-coated liposomes for oral delivery of …
Jul 1, 2003 · Polyethylene glycol (PEG)-coated liposomes containing rhEGF was prepared and evaluated for their stability and permeability in Caco-2 cells. In the animal study, we also determined plasma...
Pegylated recombinant human epidermal growth factor (rhEGF) …
Recombinant human epidermal growth factor (rhEGF) was conjugated with polyethylene glycol (PEG) to improve its physical stability during microencapsulation in biodegradable poly (lactic-co-glycolic acid) microspheres. rhEGF was conjugated with N-hydroxysuccimide (NHS)-derivatized methoxy-PEG (mPEG) of MW 2000 and 5000 under various reaction cond...
Effect of Molecular Size of PEGylated Recombinant Human …
Feb 1, 2006 · This study suggests that the high molecular weight PEG at N-terminus of rhEGF would give a satisfactory stabilizing effect and thus may improve therapeutic efficacy in clinical use.
Pegylated recombinant human epidermal growth factor (rhEGF) …
Recombinant human epidermal growth factor (rhEGF) was conjugated with polyethylene glycol (PEG) to improve its physical stability during microencapsulation in biodegradable poly (lactic-co-glycolic acid) microspheres. rhEGF was conjugated with N-hydroxysuccimide (NHS)-derivatized methoxy-PEG (mPEG) o ….
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