Enterobacteria phage T2 - Wikipedia
Enterobacteria phage T2 is a virus that infects and kills E. coli. It is in the genus Tequatrovirus, and the family Myoviridae. Its genome consists of linear double-stranded DNA, with repeats at either end. The phage is covered by a protective protein coat.
Enterobacteria Phage T2 - an overview | ScienceDirect Topics
The genome of the infecting phage brings into the bacterial cell information necessary for the production of a set of enzymes involved in the synthesis of the unusual DNA. The hydroxymethylase is one such phage-coded enzyme; a number of others are also known, including a specific kinase for hydroxymethyldeoxycytidylate, and a T2-DNA ...
Genetic Engineering of Bacteriophages Against Infectious Diseases
May 2, 2019 · This elegant experiment demonstrated that DNA, not protein, is the genetic material of T2 phage. Recently, the emergence of multi-antibiotic resistant bacterial pathogens and the low rate of new antibiotic discovery brought new urgency to develop phage-based therapies ( Lu and Koeris, 2011 ; Viertel et al., 2014 ; Domingo-Calap and Delgado ...
Enterobacteria Phage T2 - an overview | ScienceDirect Topics
Gp15 has been shown to bind DNA, and may thus escort the leading end of the phage genome into the infected cell. T7 is not unique in having three E proteins; those of P22 are also present in multiple copies in the virion.
Is phage DNA "injected" into cells - biologists and physicists can ...
We thus have a paradox; two phage genomes have been shown to be ejected into cells by a mechanism that is not controlled by pressure of the packaged DNA, whereas two others eject their genomes completely in an in vitro process where pressure of …
species of the T-even phage group (T2), the DNA genome (mw. 130 x 1()6; 166 kbp) contains 5-hydroxymethylcytosine instead of cytosine and is circularly permuted. Each molecule carries a 3% circularly permuted. Detailed genetic and physi cal maps of the genome are available. Particles adsorb to lipopolysaccharide receptors on the
Characterizing Phage Genomes for Therapeutic Applications
Apr 10, 2018 · Essential analysis checkpoints and warnings are detailed for obtaining high-quality genomes, excluding undesirable candidates, rigorously assessing a phage genome for safety and evaluating sequencing contamination.
Species Barrier Between Bacteriophages T2 and T4 ... - Oxford Academic
Apr 1, 1998 · Bacteriophage T2 alleles are excluded in crosses between T2 and T4 because of genetic isolation between these two virus species. The severity of exclusion varies in different genes, with gene 56, encoding an essential dCT (D)Pase/dUT (D)Pase of these phages, being most strongly affected.
Popping the cork: mechanisms of phage genome ejection
Feb 4, 2013 · Advances in electron microscopy have revealed the structure of the condensed DNA confined in a phage capsid, and the mechanisms and energetics of packaging a phage genome are beginning to...
High-throughput mapping of the phage resistance landscape in
Oct 13, 2020 · Here, we globally map the host genetic determinants involved in resistance to 14 phylogenetically diverse double-stranded DNA phages using two model Escherichia coli strains (K-12 and BL21) with known sequence divergence to demonstrate strain-specific differences.